Microbiology Precipitation reaction
hello everyone) I'm interested in the precipitation reaction. Namely, the purpose of its use in immunodiagnostics.
A precipitation reaction is the formation and precipitation of a complex of a soluble molecular antigen with antibodies in the form of a cloud called a precipitate. It is formed by mixing antigens and antibodies in equivalent amounts, an excess of one of them reduces the level of formation of the immune complex.
The precipitation reaction is put in test tubes (ring precipitation reaction), in gels, nutrient media, etc. Varieties of the precipitation reaction in a semi-liquid gel of agar or agarose, Ouchterlony double immunodiffusion, radium immunodiffusion, immunoepectrophoresis, etc., are widely used.
The detection of antibodies against the antigens of the pathogen in the patient's blood serum makes it possible to diagnose the disease. Serological studies are also used to identify microbial antigens, various biologically active substances, blood groups, tissue and tumor antigens, immune complexes, gut receptors, etc.
When a microbe is isolated from a patient, the pathogen is identified by studying its antigenic properties using immune diagnostic sera, i.e. blood sera from hyperimmunized animals containing specific antibodies. This is the so-called serological identification of microorganisms.
Thanks for the information, yes I agree, a fairly reliable and widely used method
@ekaterina-gribacheva Btw could the precipitation reaction be used in drug design?